Journal: Oncology reports

Article Title: Metformin inhibits proliferation and enhances chemosensitivity of intrahepatic cholangiocarcinoma cell lines.

doi: 10.3892/or.2014.3151

Figure Lengend Snippet: Figure 6. Metformin sensitizes ICC cells to certain chemotherapeutic agents and the potential mechanisms. RBE and HCCC-9810 cells were treated with a combination of metformin and one of the chemotherapeutic agents, sorafenib (sora), gemcitabine (gem), 5-fluorouracil (5-fu) and arsenic trioxide (As2O3). (A) The expression of HIF-1α protein and MRP1 protein was downregulated depending on the dose of metformin determined using western blotting. (B) The phosphorylation of ERK was downregulated depending on the dose of metformin. (C-E) All the band intensities were shown after semi-quantification using Image Lab 5.0 software and normalized with β-actin or total EKR and are represented as the means ± SD from 3 independent experiments (*P<0.05, **P<0.01). (F and G) RBE and HCCC-9810 cells were treated with sora (5 or 10 µM), gem (1.25 or 2.5 µg/ml), 5-fu (1.25 or 2.5 µg/ml) and As2O3 (1.5 or 3.0 µM), respectively, for 48 h. Then, the CCK-8 assay was used to determine cell viability. The cell viability with a combination of agent treatments was compared to the cell viability with a single chemotherapeutic agent treatment, respectively (**P<0.01).

Article Snippet: Sorafenib (C21H16ClF3N4O3C7H8O3S, CAS 475207-59-1) was purchased from Selleck Chemicals (Houston, TX, USA).

Techniques: Expressing, Western Blot, Phospho-proteomics, Software, CCK-8 Assay

Journal: Applied and Environmental Microbiology

Article Title: Bifidobacterium animalis subsp. lactis ATCC 27673 Is a Genomically Unique Strain within Its Conserved Subspecies

doi: 10.1128/AEM.01777-13

Figure Lengend Snippet: General genomic characteristics of sequenced genomes of B. animalis subspecies

Article Snippet: The recently sequenced strain Bl12 showed the most similarity with B420, with only 19 SNPs being exhibited between the two strains. table ft1 table-wrap mode="anchored" t5 caption a7 Strain Total no. of SNPs identified a DSM 10140 Bl-04 Bb-12 B420 Bi-07 BLC1 CNCM I-2494 V9 Bl12 ATCC 27673 12,053 12,666 12,679 13,187 12,861 13,160 12,322 12,600 12,558 DSM 10140 47 387 43 58 55 150 56 59 Bl-04 358 12 11 24 123 25 28 Bb-12 340 352 365 407 374 358 B420 19 123 123 21 19 Bi-07 30 139 30 34 BLC1 153 37 32 CNCM I-2494 149 142 V9 31 Open in a separate window a Total number of SNPs identified between pairs of genomes identified in the progressiveMauve program.

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: Bifidobacterium animalis subsp. lactis ATCC 27673 Is a Genomically Unique Strain within Its Conserved Subspecies

doi: 10.1128/AEM.01777-13

Figure Lengend Snippet: Total SNPs identified between strains of B. animalis subsp. lactis

Article Snippet: The recently sequenced strain Bl12 showed the most similarity with B420, with only 19 SNPs being exhibited between the two strains. table ft1 table-wrap mode="anchored" t5 caption a7 Strain Total no. of SNPs identified a DSM 10140 Bl-04 Bb-12 B420 Bi-07 BLC1 CNCM I-2494 V9 Bl12 ATCC 27673 12,053 12,666 12,679 13,187 12,861 13,160 12,322 12,600 12,558 DSM 10140 47 387 43 58 55 150 56 59 Bl-04 358 12 11 24 123 25 28 Bb-12 340 352 365 407 374 358 B420 19 123 123 21 19 Bi-07 30 139 30 34 BLC1 153 37 32 CNCM I-2494 149 142 V9 31 Open in a separate window a Total number of SNPs identified between pairs of genomes identified in the progressiveMauve program.

Techniques: